GSM4368901: Input-SIRT7 KO FKDL190746011-1a 1.fq.gz; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
Mesenchymal stem cell
cell type
Mesenchymal stem cell
genotype
SIRT7 defecient hMSCs
passage
p6
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For DamID-seq, genomic DNA were isolated using DNeasy Blood & Tissue Kit (Qiagen). For ChIP-seq, DNA was isolated sing phenol-chloroform-isoamylalcohol extraction and ethanol precipitation. For RNA-seq, total RNA was extracted in TRIzol (Thermo Fisher Scientific) and genomic DNA was removed using a DNA-free kit (Thermo Fisher Scientific) For ATAC-seq, library amplified and purified using TruePrep DNA Library Prep Kit V2 for Illumina(Vazyme Biotech). For DamID-seq, the DNA library was constructed using aNEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. For ChIP-seq, the enriched fragments were constructed into libraries without the incorporation of spike-in controls via KAPA Hyper Prep Kits with PCR Library Ampli?cation/Illumina series (KK8504) following the manufacturer's instructions. For RNA-seq, sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. DamID-seq, ATAC-seq, ChIP-seq, and RNA-seq