ChIP-Atlas: SRX7827851

Antigen

Cell type Class: Blood
Cell type: Macrophages
MeSH Description: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: Lungs were removed, single suspension cells were achieved by enzymatic digestion of tissue, and alveolar macrophage cells were harvested by FACS. Omni-ATAC was performed on 50,000 cells from two biological replicates per AM subpopulation using Tn5 transposase from the Nextera DNA Library Prep Kit (FC-121-1030). Following transposition, the Qiagen MinElute Kit (28204) was used to purify the DNA. Based on qPCR amplification curves, samples were amplified for four additional cycles after the five-cycle preamplification (9 total cycles). For size selection and clean-up of amplified library, three rounds of AMPure beads-enabled purification were performed. 1X AMPure beads were used first to remove primer dimers, followed by 0.5X to remove large fragments >1,000 kb, and finally 1.8X to further clean up.