GSM4364224: h2az mnase4 x chip rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H2A.Z
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
mouse embryonic stem cell
cell type
cell line
strain
C57BL/6
chip antibody
H2A.Z (Abcam, ab4174)
treatment
NA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were first crosslinked by formaldehyde, then digested with Mnase, then sonicated in N-lauroyl-sarcosine containing buffer to dissolve the chromatin. The clarified chromatin were immunoprecipitated with agarose conjucated with antibodies. Libraries were prepared according to NEBNext Ultra DNA Library Prep Kit for Illumina (E7370L) and were sequenced using HiSeq2000 or Nova-seq.