Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mature B
strain
C57BL/6
genotype
Pax5(fl/+) CD23-Cre
cell preparation
Ex-vivo MACS-sorted from lymphe node, CD43-

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
ATAC-seq libraries were prepared using NEBNext High-Fidelity 2x PCR Master Mix (New England Labs #M0541) and Nextera PCR primers.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
43900124
Reads aligned (%)
95.6
Duplicates removed (%)
21.5
Number of peaks
46183 (qval < 1E-05)

mm9

Number of total reads
43900124
Reads aligned (%)
95.5
Duplicates removed (%)
21.7
Number of peaks
46134 (qval < 1E-05)

Base call quality data from DBCLS SRA