Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Pax5

Cell type

Cell type Class
Blood
Cell type
Pro-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
in vitro pro-B cell
strain
C57BL/6
genotype
Wapl(deltaP1/deltaP1)
cell preparation
4-days in-vitro expansion
chip antibody
anti-Pax5 (rabbit polyclonal Ab, detecting the paired domain (amino acids 17-145) (16); Busslinger laboratory)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
33126351
Reads aligned (%)
98.2
Duplicates removed (%)
10.5
Number of peaks
31176 (qval < 1E-05)

mm9

Number of total reads
33126351
Reads aligned (%)
97.9
Duplicates removed (%)
10.5
Number of peaks
31385 (qval < 1E-05)

Base call quality data from DBCLS SRA