Antigen

Antigen Class

TFs and others

Antigen

Smc1a

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mouse embryonic stem cells

cell line

V6.5

cell line

mouse embryonic stem cells

treatment

Pol II untreated mES

chip antibody

Smc1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from MNase digested and sonicated nuclei and protein-DNA complexes were isolated with specific antibody. ChIP DNA were used to construct Illumina sequencing libraries using TELP method from (Peng et al., 2015).

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

36960277

Reads aligned (%)

51.4

Duplicates removed (%)

7.2

Number of peaks

1813 (qval < 1E-05)

mm9

Number of total reads

36960277

Reads aligned (%)

51.4

Duplicates removed (%)

7.3

Number of peaks

1788 (qval < 1E-05)