SRX7786269
GSM4333025: WT Pol2 ChIP B1T2; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic stem cells
cell line
V6.5
cell line
mouse embryonic stem cells
treatment
WT untreated mES
chip antibody
Pol2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from MNase digested and sonicated nuclei and protein-DNA complexes were isolated with specific antibody. ChIP DNA were used to construct Illumina sequencing libraries using TELP method from (Peng et al., 2015).

Sequencing Platform

instrument_model
HiSeq X Ten

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
43241389
Reads aligned (%)
63.4
Duplicates removed (%)
10.8
Number of peaks
14232 (qval < 1E-05)

mm9

Number of total reads
43241389
Reads aligned (%)
63.3
Duplicates removed (%)
10.8
Number of peaks
14172 (qval < 1E-05)

Base call quality data from DBCLS SRA