Antigen

Antigen Class

TFs and others

Antigen

BRD2

Cell type

Cell type Class

Digestive tract

Cell type

DLD-1

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

DLD-1

cell line

Colorectal adenocarcinoma DLD-1

genotype

BRD2-AID OsTIR1

chip antibody

Brd2 (D89B4) CST mAb #5848

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, 20-50 million cells were crosslinked with 1% paraformaldehyde (Electron Microscopy Sciences) in PBS for 10 min at r.t. Chromatin was sonicated with the Covaris E220 for 4 min with 10% duty cycle, 140 peak intensity power, 200 cycles per burst. Immunoprecipitations were carried out with antibodies and Protein A/G-agarose beads (Santa Cruz) or Dynabeads Protein G (Invitrogen). For ChIP-seq, DNA libraries were prepared by the HTP Library Preparation Kit for Illimina (KAPA) and sequenced on the NextSeq 500 or NovaSeq 6000 (Illumina).

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

40834116

Reads aligned (%)

95.2

Duplicates removed (%)

29.8

Number of peaks

35259 (qval < 1E-05)

hg19

Number of total reads

40834116

Reads aligned (%)

94.8

Duplicates removed (%)

30.4

Number of peaks

35496 (qval < 1E-05)