Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

B cells

cell type

Primary B cells

tissue

Freshly isolated from spleen

strain

C57BL/6

chip antibody

Acetylated H3 (Merck Millipore, 06-599)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Soluble chromatin was prepared from sonicated nuclei and the sobule chromatin was immunoprecipitated with antibodies. Libraries were prepared with NEBnext Ultra II DNA library prep kit or ThruPLEX DNA-Seq Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

38175098

Reads aligned (%)

98.5

Duplicates removed (%)

22.5

Number of peaks

17536 (qval < 1E-05)

mm9

Number of total reads

38175098

Reads aligned (%)

98.4

Duplicates removed (%)

22.7

Number of peaks

17531 (qval < 1E-05)