Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Hippocampus

MeSH Description

A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

Sample

source_name

mouse hippocampus

genotype

wild type

strain

C57/DBA F1 hybrid

tissue

hippocampus

developmental stage

adult

Sex

male

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody against H3K4me3 Millipore, #07-473). Libraries were prepared according to standard Illumina's protocols and sequenced on HiSeq 2500 platform following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

8136769

Reads aligned (%)

98.4

Duplicates removed (%)

12.9

Number of peaks

366 (qval < 1E-05)

mm9

Number of total reads

8136769

Reads aligned (%)

98.1

Duplicates removed (%)

13.1

Number of peaks

380 (qval < 1E-05)