Synchronized L1s of wild-type and mut-16(pk710) worms were plated on enriched peptone plates and cultured at 20°C and 25°C. One million animals per sample, in biological triplicates, were harvested as adults (~68hrs at 20°C and ~48hrs at 25°C). Germline nuclei were isolated as previously described (49). Nuclei were flash frozen in liquid nitrogen and stored at -80°C. For each sample, roughly 50,000 isolated germline nuclei were used. We prepared our ATAC-seq libraries as previously described (50). Library quality was assessed (Agilent BioAnalyzer Chip) and concentration was determined using the Qubit 1X dsDNA HS Assay kit (ThermoFisher Q32851). Libraries were sequenced on the Illumina NextSeq500 (PE 75-bp reads) platform. Three biological replicates were generated for wild-type (N2) and mut-16 mutants cultured at 20°C and 25°C.