Brain tissue were fixed 45' in PBS containing 2mM Di-succinimidyl-glutarate (DSG). After washing with PBS, protein-DNA-interactions were fixed by 10min incubation in 1% formaldehyde at room temperature; chromatin was fragmented to the desidered lenght with Diagenode's Bioruptor, according to manufacturer's instructions. Library construction have been performed with ChIP-Seq DNA Sample Prep Kit, according to manufacturer's instructions.