Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Embryonic brains
NA
NA

Attributes by original data submitter

Sample

source_name
Ex vivo differentiated ependymal cells
cell type
OF1
tissue
Mouse embryonic brain, lateral walls
genotype
wild type
age
P0
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated, fragmented by sonication and immunoprecipitated with anti-Rfx1 or -RFX2 or -RFX3 antibody. Libraries were prepared according to Illumina's instructions using the TruSeq ChIP-seq protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
95857497
Reads aligned (%)
91.2
Duplicates removed (%)
41.4
Number of peaks
84604 (qval < 1E-05)

mm9

Number of total reads
95857497
Reads aligned (%)
91.0
Duplicates removed (%)
41.4
Number of peaks
85072 (qval < 1E-05)

Base call quality data from DBCLS SRA