Antigen

Antigen Class

TFs and others

Antigen

Rfx1

Cell type

Cell type Class

Embryo

Cell type

Embryonic brains

NA

NA

Sample

source_name

Ex vivo differentiated ependymal cells

cell type

OF1

tissue

Mouse embryonic brain, lateral walls

genotype

wild type

age

P0

chip antibody

RFX1 antibody (Herrero-Sanchez et al., 1992, PMID: 1508204)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was isolated, fragmented by sonication and immunoprecipitated with anti-Rfx1 or -RFX2 or -RFX3 antibody. Libraries were prepared according to Illumina's instructions using the TruSeq ChIP-seq protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

43775102

Reads aligned (%)

96.7

Duplicates removed (%)

14.4

Number of peaks

6936 (qval < 1E-05)

mm9

Number of total reads

43775102

Reads aligned (%)

96.4

Duplicates removed (%)

14.4

Number of peaks

6907 (qval < 1E-05)