GSM4295204: HLE-B3 QKI KO SREBP2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SREBF2
Cell type
Cell type Class
Others
Cell type
B-3
Primary Tissue
Eye
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HLE-B3 cell line
cell line
HLE-B3
cell type
Human lens epithelial cells, transformed with an adenovirus 12-SV40 virus hybrid (Ad12-SV40)
passages
20 after received from ATCC
age
Infant less than 1 year
chip antibody
Srebp2 (10007663, Cayman Chemical)
genotype
QKI KO
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, sonicated and then incubated with Qki-5, Pol II, and Srebp2 antibodies. Protein-DNA complex were washed and ChIP'ed DNA were isolated for library preparation. Libraries were prepared by KAPA Hyper Prep Kit (KK8504, Kapa Biosystems).