GSM4294235: AGS EBF1-Q196P-EBF1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EBF1
Cell type
Cell type Class
Digestive tract
Cell type
AGS
Primary Tissue
Stomach
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
gastric cancer cell line
cell line
AGS
antibody
H00001879-M01, Abnova
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, cells were cross-linked with 1% formaldehyde for 10 min at room temperature, and reactions were stopped by adding glycine to a final concentration of 0.2 M. Chromatin was extracted and sonicated to 500 bp. EBF1 (H00001879-M01, Abnova), H3K27me3 (#07-449, Millipore), h3k27AC (ab4729, Abcam), or H3K4me1 (ab8895, Abcam) antibodies were used for chromatin immunoprecipitation (ChIP). Input DNA from cells prior to immunoprecipitation were used to normalize ChIP-seq peak calling. ChIPed DNA (10-30 ng) was used for ChIP with DNA sequencing (ChIP-seq) library construction following manufacturer protocols (New England Biolabs). For RNA-Seq, sequencing libraries were constructed from total RNA of GC cell lines with 300-400 bp insert lengths ChIP-seq, RNA-Seq