Primary pancreatic ductal adenocarcinoma tumor from KPC mouse
strain
C57BL/6
genotype
KrasLSLG12D/+; Trp53LSLR172H/LOH; Pdx1-Cre
tissue
Primary pancreatic ductal adenocarcinoma tumor
chip antibody
input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 250 bp were immunoprecipitated using H3K27ac (Abcam, ab4729) antibody. Libraries were prepared for Illumina sequencing using a standard protocol consisting in blunting, addition of dA overhangs, ligation of Illumina adapters, selection on gel and PCR with index primers.