GSM4292837: DDX43 KH 89aa 2 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293T cells
cell line
HEK293T
passage
10 to 15
ip reagent
M2-FLAG beads (A2220, Sigma)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The DNA was extracted with phenol-chloroform, precipitated with 2×volume of ethanol, washed with 70% ethanol, and dissolved in 50 µL of ddH2O. For RNA extraction and purification, Trizol reagent (Invitrogen) was used to isolate the sheared RNA. The isolated RNA was eluted in nuclease-free water. For the DNA library, 20 ng of ChIP-ed DNA was end repaired using NEBNext Ultra II End Prep buffer and Enzyme mix, adaptor-ligated, cleaned with 0.9×AMPure beads, and size selected using 0.4×beads. The size-selected DNA was then amplified using (Index 3, 4, 5, 6, 7 and 8) NEBNext Multiplex Oligos for Illumina and universal primers (E7335, NEB). The products were cleaned using 0.9×AMPure beads. The RNA library was prepared using the NEBNext Ultra II directional library prep kit (E7760, NEB) and DNA library by NEBNext ultra II DNA library prep kit (E7645, NEB) according to the manufacturer's instructions