Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Prostate

Cell type

DU 145

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

ChIPseq_H3K27me3_CTR

cell line

DU145

cell type

Human prostate cancer cell line

chip antibody

H3K27me3 (Millipore #07-449) / Drosophila H2Av (Active Motif #61686).

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin preparation and ChIP experiments were performed with the ChIP-IT High Sensitivity Kit from Active Motif (#53040) according to the manufacturer's instructions. Libraries were prepared according to Illumina instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

35551426

Reads aligned (%)

94.4

Duplicates removed (%)

5.2

Number of peaks

1133 (qval < 1E-05)

hg19

Number of total reads

35551426

Reads aligned (%)

93.6

Duplicates removed (%)

6.9

Number of peaks

1211 (qval < 1E-05)