Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Blood

Cell type

Th1 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Sample

source_name

spleen

strain

C57BL/6N

cell type

Th1 cells

treatment

DNp73 over-expression, Th1, polarized 72 hours

chip antibody

anti-FLAG (M2) antibody (Sigma)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, 10 million Th1 cells overexpressing Trp73 or vector control cells were performed using anti-FLAG (M2) antibody (Sigma) as previously described. For RNA-seq, Trp73 overexpressed Th1 cells were isolated by FACS sorting as GFP+ cells three days after retroviral transduction, and total RNA was then extracted. RNA-Seq libraries were prepared from mRNA (isolated from 2 μg total RNA) using the KAPA kit per the manufacturer's protocol. After quantification by Qubit (Invitrogen), barcoded samples were mixed and sequenced on an Illumina HiSeq2000 or HiSeq3000 system.

Sequencing Platform

instrument_model

Illumina HiSeq 3000

mm10

Number of total reads

69581343

Reads aligned (%)

98.3

Duplicates removed (%)

18.8

Number of peaks

8230 (qval < 1E-05)

mm9

Number of total reads

69581343

Reads aligned (%)

98.1

Duplicates removed (%)

18.8

Number of peaks

8272 (qval < 1E-05)