GSM4289308: Cardiff CTL28n6 14 C 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived neural cells
NA
NA
Attributes by original data submitter
Sample
source_name
Control Sample H3K4me3 ChIP-Seq
subject status
control
cell type
iPSC-derived medium spiny neuron-like cells
chip antibody
H3K4me3 (Millipore, 07-473)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was digested with 150 units of MNase (NEB, M0247S) for 10min at 37°C. The digestion reaction was stopped with 10mM EDTA. The chromatin was incubated with 1ul of k4me3 (Millipore, 07-473) overnight and 20ul of Protein G beads (ThermoFisher, 10004D) were added for 2hr incubation at 4°C on the following day Immunoprecipitated DNA and nonspecific IgG-bound DNA were prepared for high-throughput sequencing according to Illumina's instructions and sequenced on an Illumina platform following the manufacturer's standard protocol.