Lysates were clarified from sonicated nuclei and PPARs-DNA complexes were isolated with antibody. Extracted DNA (1-5ng) was used to prepare sequencing libraries using the NEBNext ChIP-seq library preparation master mix set for Illumina (New England Biolabs, E6240S). The library was analyzed by Agilent Bioanalyzer and was sequenced by the Functional Genomics Core of the Penn Diabetes Research Center by using the Illumina HiSeq2500 (single-end 50bp)