Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Neural

Cell type

Retina

MeSH Description

The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.

Sample

source_name

Retina

strain

C57BL/6

genotype/variation

Chx10-Cre Rb1/p107

tissue

Retina EGFP+ cells

age

P21

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Approximately 50,000 cells were harvested for ATAC-seq for each replicate. Briefly, retinae were dissociated, flow sorted into EGFP- or EGFP+, assessed for cell viability, counted, and washed with PBS. ATAC-seq was performed as previously described (Buenrostro et al. 2015, Nature 523:486-490).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

44562184

Reads aligned (%)

64.5

Duplicates removed (%)

11.8

Number of peaks

19663 (qval < 1E-05)

mm9

Number of total reads

44562184

Reads aligned (%)

64.5

Duplicates removed (%)

11.9

Number of peaks

19612 (qval < 1E-05)