GSM4286005: EC Patient 08 ATACseq Central memory CD4 T cells (2); Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
Central memory CD4 T cells
patient
EC Patient 08
tissue
peripheral blood
replicate
2
cell type
Central memory CD4 T cells
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
DNA was treated with Tn5 and purified using Chip dna clean & concentrator 20,000 sorted cells were centrifuged at 1500 rpm for 10 min at 4°C in a pre-cooled fixed-angle centrifuge. All supernatant was removed and a modified transposase mixture (including 25 µl of 2x TD buffer, 1.5 µl of TDE1, 0.5 µl of 1% digitonin, 16.5 µl of PBS, 6.5 µl of nuclease-free water) was added to the cells and incubated in a heat block at 37°C for 30 min. Transposed DNA was purified using a ChIP DNA Clean & Concentrator Kit (Zymo Research #D5205) and eluted DNA fragments were used to amplify libraries.