Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GFP

Cell type

Cell type Class
Larvae
Cell type
L3
NA
NA

Attributes by original data submitter

Sample

source_name
L3
tissue
larvae
strain
egl43-gfp
Stage
L3
chip antibody
anti-GFP
chip antibody supplier
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitations and library preparations were conducted as in (McMurchy et al., 2017), using formaldehyde and EGS as fixatives and the anti-GFP antibody ab290 (Abcam) to precipitate chromatin-bound EGL-43::GFP.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
11433374
Reads aligned (%)
69.5
Duplicates removed (%)
18.5
Number of peaks
5425 (qval < 1E-05)

ce10

Number of total reads
11433374
Reads aligned (%)
69.5
Duplicates removed (%)
18.5
Number of peaks
5296 (qval < 1E-05)

Base call quality data from DBCLS SRA