Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Blood
Cell type
P5424
NA
NA

Attributes by original data submitter

Sample

source_name
P5424 H3K4me1 (DMSO)
cells used
2.00E+06
antibody
H3K4me1
antibody quantity
3 µg
magnetic beads
Protein G dynabeads
bead volume
20µl
estimated fragment size
196
cell line
P5424

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Phenol-chroloform Library preparation was carried out as per manufacturer’s instructions (Illumina) using 10ng material. Samples were subsequently size-selected via gel migration and extraction, with average cut sizes at about 230 bp, including adapters. Sequencing was carried out on an Illumina Genome II Analyzer. The quality of libraries was checked with the Bioanalyser DNA High sensitivity and by Q-PCR. Sequencing was performed with the

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
48787435
Reads aligned (%)
87.2
Duplicates removed (%)
40.5
Number of peaks
47510 (qval < 1E-05)

mm9

Number of total reads
48787435
Reads aligned (%)
87.1
Duplicates removed (%)
40.6
Number of peaks
47572 (qval < 1E-05)

Base call quality data from DBCLS SRA