GSM1548404: P5424 RNA Pol II (KM) ChIP-Seq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Blood
Cell type
P5424
NA
NA
Attributes by original data submitter
Sample
source_name
P5424 RNA Pol II (KM)
cells used
1.00E+07
antibody
RNA Pol II
antibody quantity
10 µg
magnetic beads
Protein G dynabeads
bead volume
50µl
estimated fragment size
170
cell line
P5424
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Phenol-chroloform Library preparation was carried out as per manufacturer’s instructions (Illumina) using 10ng material. Samples were subsequently size-selected via gel migration and extraction, with average cut sizes at about 230 bp, including adapters. Sequencing was carried out on an Illumina Genome II Analyzer. The quality of libraries was checked with the Bioanalyser DNA High sensitivity and by Q-PCR. Sequencing was performed with the