Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1 human embryonic stem cells (WA01)
tissue
H1 human embryonic stem cells (WA01)
culture
mTESRI, matrigel

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared according to Illumina's instructions Chromatin was prepared from 3.10E+7 fixed H1 cells sonicated with Covaris Focus ultra-sonicator and KZFP-DNA complexes were isolated by immunoprecipitation with anti-HA antibody

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
31885349
Reads aligned (%)
96.7
Duplicates removed (%)
24.9
Number of peaks
1340 (qval < 1E-05)

hg19

Number of total reads
31885349
Reads aligned (%)
95.9
Duplicates removed (%)
25.4
Number of peaks
901 (qval < 1E-05)

Base call quality data from DBCLS SRA