Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Prostate

Cell type

22Rv1

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

Human prostate cell line 22Rv1

tissue

human prostate cancer

treatment

None

genotype

wild type

cell line

22Rv1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted using the DirectZol RNA kit (Zymo, R2062). The RNA_seq libraries were made using Kapa mRNA HyperPrep Kit (KAPA, KKK8581) according to manufacturer's instructions. ChIP_seq libraries were prepared using the Kapa Hyper prep kit (Kapa, KK8503) according to the manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

30561782

Reads aligned (%)

99.0

Duplicates removed (%)

8.2

Number of peaks

1124 (qval < 1E-05)

hg19

Number of total reads

30561782

Reads aligned (%)

98.2

Duplicates removed (%)

9.6

Number of peaks

1201 (qval < 1E-05)