Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Bach2

Cell type

Cell type Class
Blood
Cell type
Naive T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Naïve CD4 T cells
strain
C57BL/6
genotype
Bach2 KO
tissue
spleen
cell type
CD4 T cells
cell cultured conditions
Thn conditions (IL-2, anti-IFN-g, anti-IL-4)
chip antibody
Bach2 (SIGMA, cat#: SAB2001201, lot#: QC10599

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and transcription factor- or histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the ChIP Sample Prep Kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
28465558
Reads aligned (%)
90.7
Duplicates removed (%)
25.1
Number of peaks
7063 (qval < 1E-05)

mm9

Number of total reads
28465558
Reads aligned (%)
90.5
Duplicates removed (%)
25.2
Number of peaks
7024 (qval < 1E-05)

Base call quality data from DBCLS SRA