Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Lung

Cell type

PC-9

Primary Tissue

Lung

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

lung cancer cell

antibody

H3K4me2

antibody vendor

Abcam

antibody catalog

ab7766

cell type

lung cancer cell

passages

8-12

cell line

PC9

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

mRNA were extracted by Tiangen kits (#DP431 ) and whole DNA were extracted by Qiagen DNeasy 96 Blood & Tissue Kit kits, and ChIP antibody were from Abcam Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

75465456

Reads aligned (%)

99.3

Duplicates removed (%)

7.0

Number of peaks

72362 (qval < 1E-05)

hg19

Number of total reads

75465456

Reads aligned (%)

99.0

Duplicates removed (%)

7.5

Number of peaks

72252 (qval < 1E-05)