Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Spi1

Cell type

Cell type Class
Blood
Cell type
X18.1.1
NA
NA

Attributes by original data submitter

Sample

source_name
X18.1.1 AML cell line, empty vector, Pu.1 ChIP
cell line
X18.1.1
cell type
AML cell line
transfection
empty vector
chip antibody
Pu.1 (Santa Cruz sc352x)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Illumina HiSeq 2000

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
17992486
Reads aligned (%)
83.0
Duplicates removed (%)
14.5
Number of peaks
5415 (qval < 1E-05)

mm9

Number of total reads
17992486
Reads aligned (%)
82.8
Duplicates removed (%)
14.6
Number of peaks
5426 (qval < 1E-05)

Base call quality data from DBCLS SRA