Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Neural
Cell type
Cortical neuron
NA
NA

Attributes by original data submitter

Sample

source_name
mouse primary cortical neuron
antibody
Anti- M2 Sigma (Cat. No. A2220)
passages
R26/LSD1c DIV10
cell type
mouse primary cortical neuron
treatment
KCl
time
1 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated nuclei were precleared with Protein G magnetic beads and antibody chromatin complexes were isolated, and eluted DNA was reverse cross-linked overnight and DNA was purified using Qiagen DNA purification columns using Manufacturer's recommended protocol. Libraries were prepared using Illumina's recommended protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
25779785
Reads aligned (%)
92.0
Duplicates removed (%)
5.2
Number of peaks
394 (qval < 1E-05)

mm9

Number of total reads
25779785
Reads aligned (%)
91.9
Duplicates removed (%)
5.2
Number of peaks
381 (qval < 1E-05)

Base call quality data from DBCLS SRA