Antigen

Antigen Class

Histone

Antigen

H4K20me1

Cell type

Cell type Class

Neural

Cell type

Cortical neuron

NA

NA

Sample

source_name

mouse primary cortical neuron

antibody

Anti- H4K20me1 Abcam (Cat. No. ab9051)

passages

LSD1n WT DIV10

cell type

mouse primary cortical neuron

treatment

KCl

time

1 hours

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclei were precleared with Protein G magnetic beads and antibody chromatin complexes were isolated, and eluted DNA was reverse cross-linked overnight and DNA was purified using Qiagen DNA purification columns using Manufacturer's recommended protocol. Libraries were prepared using Illumina's recommended protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

52318839

Reads aligned (%)

97.7

Duplicates removed (%)

8.1

Number of peaks

480 (qval < 1E-05)

mm9

Number of total reads

52318839

Reads aligned (%)

97.5

Duplicates removed (%)

8.1

Number of peaks

581 (qval < 1E-05)