Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Foxo1

Cell type

Cell type Class
Cardiovascular
Cell type
Heart
MeSH Description
The hollow, muscular organ that maintains the circulation of the blood.

Attributes by original data submitter

Sample

source_name
Heart Tissue
tissue
heart
genotype
Wild Type
surgical procedure
Hypertrophied Heart: 12 wk old, male mice were subjected to subcutaneous isoproterenol injections (3 mg/kg/day) for 7 days
days post surgery
7
strain
C57BL/6
antibody
Anti-FoxO1 (Santa Cruz Biotechnology- sc-11350)
Sex
male

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After 7 days, hearts were removed, snap frozen, and shipped to Active Motif (a pool of 3 hearts per condition). Libraries were constructed by Active Motif (https://www.activemotif.com/).

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
40442887
Reads aligned (%)
78.3
Duplicates removed (%)
51.5
Number of peaks
4790 (qval < 1E-05)

mm9

Number of total reads
40442887
Reads aligned (%)
78.2
Duplicates removed (%)
51.5
Number of peaks
4761 (qval < 1E-05)

Base call quality data from DBCLS SRA