Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

HBL-1

Primary Tissue

Blood

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Lymphoma

Sample

source_name

HBL-1 ABC-DLBCL B cells

cell type

Activated B Cell like Diffuse Large B Cell Lymphoma cell line

chip antibody

preimmune rabbit IgG (Bethyl Labs P120-101)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

13411026

Reads aligned (%)

47.0

Duplicates removed (%)

4.0

Number of peaks

85 (qval < 1E-05)

hg19

Number of total reads

13411026

Reads aligned (%)

46.3

Duplicates removed (%)

4.1

Number of peaks

80 (qval < 1E-05)