Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Embryo

Cell type

Palate

MeSH Description

The structure that forms the roof of the mouth. It consists of the anterior hard palate (PALATE, HARD) and the posterior soft palate (PALATE, SOFT).

Sample

source_name

E12.5_Meis2_ChIP

strain background

C57BL/6

development stage

E12.5

genotype/variation

Meis2-HA

tissue/cell type

anterior palate mesenchyme

chip antibody

Anti-HA tag antibody (Abcam, ab9110)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The anterior palate mesenchyme from E12.5 Meis2HA mouse embryos was fixed in 1% formaldehyde for 10 min and quenched by glycine for 5 min at room temperature. After washing with ice-cold PBS containing cocktail protease inhibitor, tissues were dispersed into single cell suspension on ice. ChIP-seq DNA Library Prep Kit for Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

58361431

Reads aligned (%)

72.7

Duplicates removed (%)

7.5

Number of peaks

634 (qval < 1E-05)

mm9

Number of total reads

58361431

Reads aligned (%)

72.6

Duplicates removed (%)

7.5

Number of peaks

594 (qval < 1E-05)