expressing AR-V7 in response to doxycycline (LNCaP AR-V7)
treatment
10uM R1881 for 24 hrs
chip antibody
AR antibody (RRID: AB_2793341)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Biological replicates of LNCaP AR-V7 cells treated with 10uM R1881 or 20ng/mL for 24 hours were formaldehyde fixed and and pooled together. Nuclear extracts were prepared in a water bath sonicator and DNA-protein complexes were isolated with AR antibody and Protein A/G Magnetic Agarose. ChIP-exo libraries were prepared using ChIP-exo Kit from ActiveMotif following manufacturer's instructions. Libraries were sequenced on Illumina HiSeq 2500 following the manufacturer's protocols. Libraries were constructed using ChIP-exo library preparation kit (ActiveMotif, USA) following the manufacturer's instructions. cDNA libraries were sequenced on the Illumina HiSeq 2500 platform by ActiveMotif, USA.