Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Myeloid Cells

MeSH Description

The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).

Sample

source_name

myeloid cells

cell type

Myeloid cells

genotype/variation

WT

differentiation time

96h

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Nuclei harvested from 100,000 cells per sample. Each sample of the same genotype/timepoint is a biologically independent replicate (n=3 biological replicates). Direct library construction by the Omni-ATAC protocol, in which cell nuclei were incubated directly with transposase.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

24563011

Reads aligned (%)

93.5

Duplicates removed (%)

37.5

Number of peaks

26631 (qval < 1E-05)

mm9

Number of total reads

24563011

Reads aligned (%)

93.4

Duplicates removed (%)

37.9

Number of peaks

26590 (qval < 1E-05)