Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Blood

Cell type

Myeloid Cells

MeSH Description

The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).

Sample

source_name

myeloid cells

cell type

Myeloid cells

genotype/variation

HMGN1 over expression

differentiation time

96h

mnase units

15u

chip antibody

H3

replicate

Replicate 1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysis and chromatin digestion with MNase (15 units or 60 units) as described in the Mint-ChIP3 protocol on https://www.protocols.io/view/mint-chip3-a-low-input-chip-seq-protocol-using-mul-wbefaje T7 adapter ligation, linear amplification, reverse transcription and PCR as described in the Mint-ChIP3 protocol.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

1425911

Reads aligned (%)

92.5

Duplicates removed (%)

9.7

Number of peaks

43 (qval < 1E-05)

mm9

Number of total reads

1425911

Reads aligned (%)

92.3

Duplicates removed (%)

10.1

Number of peaks

44 (qval < 1E-05)