GSM4272557: siNT2 ATAC-seq rep1; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Muscle
Cell type
RMS13
NA
NA
Attributes by original data submitter
Sample
source_name
skeletal muscle
cell line
RMS13
sirna
non-targeting
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
DNA for ATAC-seq was prepared from 20,000 cells following the OMNI-ATAC procedures from Corces et al. (Nature Methods 2017) and Buenrostro et al. (Curr Protoc Mol Biol 2016), with modifications, using the Nextera kit (Illumina). The cells were lysed for 3 minutes on ice and transposed for 30 minutes at 37C. DNA was cleaned-up using procedures of a DNA extraction column. The DNA libraries were prepared with 5-9 cycles of PCR amplification with the NEB High Fidelity Master Mix (New England Biolabs). Clean up was done using the Zymo DNA Clean and Concentrator kit (Zymo Research) and followed with AMPure XP (Beckman Coulter) bead cleanup to remove primer dimers and under-digested chromatin.