Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa cells

cell line

HeLa

cell type

epithelial

disrupted gene

Periphilin

antibody

anti-H3K9me3 Abcam ab8898

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinking: 1% formaldehyde; Shearing: Bioruptor (Diagenode) to target size of 300bp; IP: 5ug primary antibody overnight Illumina TruSeq ChIP Sample Prep Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

83133572

Reads aligned (%)

96.1

Duplicates removed (%)

13.5

Number of peaks

5848 (qval < 1E-05)

hg19

Number of total reads

83133572

Reads aligned (%)

93.8

Duplicates removed (%)

16.5

Number of peaks

4517 (qval < 1E-05)