GSM4271097: 20180703 d5 KO 40ugRUNX3 IDX782; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Runx3
Cell type
Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
OT-I CD8 T cells
strain background
OT-I transgenic
hdac3 genotype
KO
cell type
CD8 T cells
drug treatment
NIL
day post-activation
Day 5
chip antibody
RUNX3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed using 1% formaldehyde and chromatin sheared to 300-500 bp in size using the Covaris E220 ultrasonicator. Resulting chromatin was incubated overnight with indicated antibodies. Purified immunoprecipitates were isolated and quantified by Qubit fluorometer. DNA sequencing libraries were prepared using the ThruPLEX-FD Prep kit (Rubicon Genomics). Libraries were sequenced using 75-bp reads on the Illumina platform at the Dana-Farber Cancer Institute.