Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

whole adult testis

strain

B6xCAST

tissue

testis

prdm9 genotype

HUM/CAST

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Testes samples were stripped of the tunica and processed as described in Khil et al, Genome Research, 2012). Single-stranded DNA sequencing and double-strand break detection were performed using a modified ssDNA ChIPseq protocol. Full experimental procedures are reported in accompanying manuscript (Hinch et al). Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

89693722

Reads aligned (%)

42.5

Duplicates removed (%)

31.2

Number of peaks

8217 (qval < 1E-05)

mm9

Number of total reads

89693722

Reads aligned (%)

42.4

Duplicates removed (%)

31.3

Number of peaks

8214 (qval < 1E-05)