Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Prostate

Cell type

RWPE-1

Primary Tissue

Prostate

Tissue Diagnosis

Normal

Sample

source_name

normal prostate epithelial cells immortalized with HPV18

cell line

RWPE-1

passage

< 20

antibody/enzyme

H3K4me1 (Abcam, ab8895, lot GR114264-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The genomic DNA was extracted following Dnase digestion (Dnase), Chromatin Immunoprecipitation following sonication (H3K27ac, H3K27me3, H3K36me3, H3K4me1, H3K4me3) or simply following sonication (Input). Libraries were constructed using the Rubicon Thruplex FD kit (Rubicon cat# R40048) according to the manufacturer's recommended protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

27678783

Reads aligned (%)

89.9

Duplicates removed (%)

74.5

Number of peaks

7630 (qval < 1E-05)

hg19

Number of total reads

27678783

Reads aligned (%)

89.7

Duplicates removed (%)

74.8

Number of peaks

7577 (qval < 1E-05)