GSM4260063: iLSC-RUNX1 KD-rep3 [ATAC-seq]; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA
Attributes by original data submitter
Sample
source_name
human iPSCs
genotype/variation
RUNX1 KD
differentiation stage
Day 18 of hematopoietic differentiation
time post infection
48 hours after transduction
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
nuclei were isolated by washing with ATAC buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2) and lysing with 50 ul of ATAC lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40). 1ml of ATAC buffer was added to dilute lysis buffer Cell lysates were spun to obtain nuclear pellets, which were subjected to transposase reaction using the Illumina Nextera DNA Sample Preparation Kit according to the manufacturer's instructions. The reaction was stopped by adding SDS to a final concentration of 0.2%.