GSM4259650: ATACseq Sub5 GF rep1; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Adipocyte
Cell type
Subcutaneous adipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
subcutaneous gluteofemoral fat
body type
apple
cell type
adipocytes
fat depot
subcutaneous gluteofemoral fat
Sex
female
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Fat biospsies were collected, adipocytes were isolated by centrifugation. Nuclei were used for tagmentation using Nextera DNA Library Preparation Kit (Illumina).DNA was purified with MinElute PCR Purification Kit (Qiagen). Tagmented DNA was amplified with Kapa HiFi Hot Start Kit (Kapa Biosystems) using 16 PCR cycles. Amplified libraries were purified with Agencourt AMPure XP (Beckman Coulter). Fragment distribution of libraries was assessed with Agilent Bioanalyzer.