GSM4259529: MCF7 E2 siYTHDC1 BRD4-1 ChIP-Seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7
cell line
MCF7
cell type
human breast cancer cell line
treatment
E2 60 mins,siYTHDC1
antibody
BRD4,Bethyl,A301-985A100
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and chromatin were isolated with BRD4 antibody. DNA libraries were prepared using NEB Next Ultra II DNA library prep kit for illumina according to manufactorer's instructions