Antigen

Antigen Class

Histone

Antigen

H2AK119ub

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mouse embryonic stem cell

treatment

untreated

antibodies

Cell Signaling (D27C4) #8240

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-Seq samples: phenol-chloroform extraction For ChIP-Seq samples: Libraries were prepared using the NEXTflex ChIP-Seq kit (Bio Scientific) following the “No size-selection cleanup” protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

32432974

Reads aligned (%)

96.6

Duplicates removed (%)

9.2

Number of peaks

288 (qval < 1E-05)

mm9

Number of total reads

32432974

Reads aligned (%)

96.5

Duplicates removed (%)

9.2

Number of peaks

255 (qval < 1E-05)