Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NOTCH1

Cell type

Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Hematopoietic progenitor cells
cell type
CD34+ cells
expansion
6 days
chip antibody
Notch

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Notch antibody bound genomic DNA complexes Libraries were prepared as follows: DNA was end-repaired using End-It DNA Repair kit (Epicenter ER0720). The blunt, phosphorylated ends were treated with Klenow and dATP for ligation of Illumina's adapters. After adapter ligation DNA was PCR amplified with Illumina primers for 18 cycles and library fragments of ~250 bp were gel extracted and purified. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina Hi-seq

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
21245862
Reads aligned (%)
15.7
Duplicates removed (%)
79.7
Number of peaks
1165 (qval < 1E-05)

hg38

Number of total reads
21245862
Reads aligned (%)
16.5
Duplicates removed (%)
78.8
Number of peaks
1171 (qval < 1E-05)

Base call quality data from DBCLS SRA