GSM1537955: Angptl2-Notch ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NOTCH1
Cell type
Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
Hematopoietic progenitor cells
cell type
CD34+ cells
expansion
6 days
chip antibody
Notch
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Notch antibody bound genomic DNA complexes Libraries were prepared as follows: DNA was end-repaired using End-It DNA Repair kit (Epicenter ER0720). The blunt, phosphorylated ends were treated with Klenow and dATP for ligation of Illumina's adapters. After adapter ligation DNA was PCR amplified with Illumina primers for 18 cycles and library fragments of ~250 bp were gel extracted and purified. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina Hi-seq